@A MyLupusTeam Member
Fascinating! Yes, negative ANA Lupus is rare and you’d think it would be impossible to have a positive dsDNA with a negative ANA! But obviously it happens. Which makes me wonder if it’s because of the inaccuracies of the Elisa testing!

The different between negative and 1:320 is pretty damn huge! Yet this happened on the very same blood sample. I find that inexcusable quite honestly!

Had my 1st test been 1:320 my rheumatologist would have acted much more quickly! Instead it was 1:80 via Elisa and he had a wait and see attitude. That wasn’t good enough for me so I went to gf a 2nd opinion I tested at the 1:320 IFA and we sprung into action based on my test results and symptoms. But how many people are dismissed because of the poor results with this Elisa test

The research study I read said even after being confronted with the truth about Elisa doctors still didn’t want to change over to IFA because of the cost. They figured if a person was sick they eventually get treatment anyway 😳 But for the patient those wasted weeks, months or years and the risk of being dismissed when feeling so ill is inexcusable

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A MyLupus...

I have a consistent positive anti dsdna but my ana was only really positive the fist time I had it tested. I suspect that the rheumatologist who tested me the first time did the IFA ana test and the subsequent test have been done with Elisa. It should not be possible to have a positive dsdna and a negative ana. However it doesn't seem to bother any doctors. I have a theory though. Elisa is a cell based assay. Cells contain phospholipids. I have antiphospholipids (antibodies against phospholipids). If the antiphospholipids binds to the phospholipids in the cells in the Elisa assay it might hinder the binding of the ana (ex. Anti dsdna antibodies) resulting in a false negative result.

Thanks for this info. I had not heard about different tests.

My ana was positive from beginning. I started this with extreme pain everywherebon body. Every joint muscles tissues was at a 10 for pain. I was 19 at the timev6 months later lupus was diagnosed. It took 6 months for labs and drs. To diagnose but the ana is main test if that comes back positive you have this disease. My best to you dear. I've lived with this since then i have had two children but the lupus caused me lots of problens with my pregnancies before the children God blessed me with. This disease is complicated. You need to stay educated and informed take your drs. Advise pay attention to your body.

Thank you for sharing. I usually get tested with Elisa, really interesting research, the results come and go, positive and negative.